Product description
Product Name: Lysozyme Cell Lysis Buffer for Gram-Positive Bacteria
Product Summary
The Lysozyme Buffer is a specialized, crucial component in the workflow for Genomic DNA (gDNA) extraction from Gram-Positive Bacteria. Unlike Gram-negative bacteria, Gram-positive species possess a thick, rigid Peptidoglycan Layer in their cell wall, which makes them highly resistant to standard chemical or enzymatic lysis methods.
Key Features and Specifications
Genomic DNA Extraction:
Pre-Lysis Step: Used as the first incubation step in gDNA isolation protocols to break down the cell wall before adding standard lysis reagents (like Proteinase K or detergents).
Challenging Samples: Crucial for research involving environmental or clinical isolates of Gram-positive organisms (e.g., Staphylococcus aureus or Clostridium species) where complete cell lysis is difficult.
Molecular Biology:
PCR and Sequencing: Ensures the yield of highly pure gDNA, free from contaminants that could inhibit downstream molecular reactions such as PCR amplification, cloning, and Next-Generation Sequencing (NGS) library preparation.
Research & Development:
Strain Characterization: Used to prepare genomic DNA for genotyping, phylogenetic analysis, and molecular identification of bacterial strains.
Usage Protocol Notes
Incubation: Requires an optimized incubation time (typically 30–60 minutes) and temperature (usually 37°C) to allow the Lysozyme enzyme sufficient time to digest the peptidoglycan layer effectively.
Storage: Lysozyme is a temperature-sensitive enzyme. The buffer or the reconstituted enzyme should be stored at the recommended low temperature ( or ) to maintain its enzymatic activity.
