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Nutrient Agar Culture Medium (Biochemical Reagent)

Product description

Product Name: Nutrient Agar (NA) Dehydrated Culture Medium

Key Features and Specifications

Feature CategorySpecification
TypeGeneral-Purpose, Non-Selective Basal Medium
FunctionCultivation and Enumeration of a wide variety of non-fastidious microorganisms (Bacteria and Yeast)
FormulationContains Peptone (source of nitrogen and amino acids), Beef Extract (vitamins, minerals), and Agar (solidifying agent)
FormatDehydrated Powder (Ready-to-use after hydration and sterilization)
pH (Final)Typically 7.4 0.2 (Optimal for most bacteria)
Sterilization MethodAutoclavable (Typically 121°C for 15 minutes)
Key AdvantageBroad-Spectrum Support for standard laboratory procedures; excellent for routine plating and maintenance.

Product Summary

Nutrient Agar (NA) is one of the most fundamental and universally used culture media in the Biochemical / Life Sciences and microbiology sectors. It is a basal medium, meaning it provides the basic nutritional requirements necessary to support the growth of a broad spectrum of non-fastidious (non-picky) microorganisms.

It’s simple formulation—primarily water, a protein digest source (peptone), beef extract, and agar—makes it the standard choice for routine subculturing, plate counting, and the storage of bacterial strains. It is a non-selective medium, allowing almost all bacteria present in a sample to grow, making it ideal for initial screening and enumeration studies.

Primary Applications

  1. Microbiology & Bacteriology:

    • General Cultivation: Used for the routine growth and maintenance of common bacterial strains (e.g., E. coli, B. subtilis).

    • Enumeration/Plate Counting: Essential for determining the total number of viable microorganisms in a sample (e.g., food, water, air) using the standard plate count method.

    • Sample Isolation: Initial streaking of environmental, clinical, or food samples to obtain isolated colonies for further characterization.

  2. Quality Control (QC):

    • Sterility Testing: Used as a reference medium to confirm the sterility of equipment, media, or products where microbial contamination is a concern.

  3. Educational & Training Laboratories:

    • Due to its broad applicability and simple preparation, Nutrient Agar is the medium of choice for introductory microbiology experiments and student training.

Preparation and Use

  • Convenience: Supplied as a dehydrated powder, requiring only the addition of purified water and sterilization by autoclaving before being poured into Petri dishes or dispensed into tubes.

  • Storage: Dehydrated media should be stored in a cool, dark, and dry place to maximize shelf life. Poured plates should be stored refrigerated (2-8°C) to maintain moisture.

  • Customization: Nutrient Agar can be supplemented with specific additives (e.g., blood, specialized sugars, or selective agents) to create enriched or selective media for more specialized microbial studies.

Color

Blue

Size

30, 32, 34, 36

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